The new SproutAngio tool provides an automated and accurate tool for the quantitation of angiogenic sprouts and vessel structures from bioimages. SproutAngio is open-source, accessible, and approachable to all researchers without programming experience.
Circular Vectors better than plasmids for RNA guide delivery and expression in cell culture experiments
We have developed an efficient, fully synthetic method and protocol called the Circular Vector method, which enables the production of circularized DNA containing expression elements that are ready for transfection in as little as 3 hours, thereby eliminating all bacterial cloning steps.
Ultraflat graphene membrane is developed here as the supporting film for cryo-EM specimen preparation, which improves the image quality of vitrified samples and enables the high-resolution reconstruction of small macromolecules (<100KD).