We have detailed a method for profiling DNA methylation and chromatin accessibility state from a single fragment library. This joint-profiling method, termed ATAC-Me, can now be applied in diverse cellular models to shed light on the complicated relationship between these two epigenetic features.

In our Nature Protocol, we present step by step instructions on how to make phosphatase inhibitor beads, enrich lysates for phosphoprotein phosphatases, multiplex, phosphopeptide enrich, and analyze samples by mass spectrometry to systematically study the major drivers of protein dephosphorylation.

Single cell Hi-C (scHi-C) maps chromatin spatial organization at single cell resolution, but the sparsity of the resulting data presents significant challenges in data analysis. We report a novel method, SnapHiC, which detects chromatin loops from scHi-C data at high sensitivity and specificity.

Raman spectroscopy is an essential technique of process analytical technologies used in the chemical and biopharmaceutical industry for uninterrupted monitoring of manufacturing processes. Now, it is also a powerful tool for monitoring solid-state milling reactions on vibratory ball mills.

Early detection of cancer is key to better outcomes for patients, and cancer screening using minimally invasive samples such as blood, urine, saliva and exfoliated cells is receiving increasing attention.