Multiplexed DNA fluorescence in situ hybridization (FISH) imaging technology has been developed to map the folding of chromatin fibers at tens of nanometers and several kilobases in resolution in single cells. We present SnapFISH to process the multiplexed DNA FISH data and identify chromatin loops.
We provided adequate data to support the crucial role of IL-18 in the development of EoE and showed the pretreatment with NLRP3 and caspase-1 inhibitors such as MCC950, BHB, and VX-765 protects against both food allergen- and aeroallergen-induced EoE pathogenesis in an EoE experimental model.
We engineered a novel family of genetically-encoded fluorescent norepinephrine indicators in green and red. These indicators exhibit exceptional sensitivity, ligand-specificity, and temporal resolution, surpassing prior indicators and enabling the detection of norepinephrine in living animals.
How is the identity of a cell encoded in its genome? And what underlies transcriptomic changes in disease states? SCENIC+ helps researchers provide answers to these questions (and many more … ) by inferring enhancers and gene regulatory networks.
Prioritized analysis enables proteins of interest to be consistently quantified across single-cell samples at no cost to depth of coverage. Applied to primary macrophages, prioritization facilitated the quantification of proteolytically regulated proteoforms and the study of endocytic competency.
Finding the hidden needle: a workflow for 3D cryo-correlative light and electron microscopy volume imaging
Imagine capturing 3D images of whole tissue in an unperturbed hydrated state, transitioning from 3D cryo-light microscopy to 3D cryo-electron microscopy. Now this is possible with our 3D cryoCLEM workflow.
Measuring FRET on samples with high or spatially varying autofluorescence and/or low (physiological) expression level? No need to fret anymore!