Behind the Paper
The real stories behind the latest research papers, from conception to publication, the highs and the lows
SnapFISH: a computational pipeline to identify chromatin loops from multiplexed DNA FISH data
Multiplexed DNA fluorescence in situ hybridization (FISH) imaging technology has been developed to map the folding of chromatin fibers at tens of nanometers and several kilobases in resolution in single cells. We present SnapFISH to process the multiplexed DNA FISH data and identify chromatin loops.
New insight into the onset, progression, and treatment of eosinophilic esophagitis (EoE)
We provided adequate data to support the crucial role of IL-18 in the development of EoE and showed the pretreatment with NLRP3 and caspase-1 inhibitors such as MCC950, BHB, and VX-765 protects against both food allergen- and aeroallergen-induced EoE pathogenesis in an EoE experimental model.
Designing next-generation norepinephrine indicators for in vivo imaging
We engineered a novel family of genetically-encoded fluorescent norepinephrine indicators in green and red. These indicators exhibit exceptional sensitivity, ligand-specificity, and temporal resolution, surpassing prior indicators and enabling the detection of norepinephrine in living animals.
Increasing single-cell data completeness and depth of coverage with prioritized proteomics
Prioritized analysis enables proteins of interest to be consistently quantified across single-cell samples at no cost to depth of coverage. Applied to primary macrophages, prioritization facilitated the quantification of proteolytically regulated proteoforms and the study of endocytic competency.
Finding the hidden needle: a workflow for 3D cryo-correlative light and electron microscopy volume imaging
Imagine capturing 3D images of whole tissue in an unperturbed hydrated state, transitioning from 3D cryo-light microscopy to 3D cryo-electron microscopy. Now this is possible with our 3D cryoCLEM workflow.
