An open access journal from Nature Portfolio publishing high-quality research, reviews and commentary in all areas of the biological sciences, representing significant advances and bringing new biological insight to a specialized area of research.
Super-resolution volume imaging realized by scanning single molecule localization microscopy (scanSMLM)
Scanning SMLM is probably the simplest, hassle-free, easy-to-use, economical, and most reliable technique for upgrading an existing SMLM to a 3D volume super-resolution system.
We provided adequate data to support the crucial role of IL-18 in the development of EoE and showed the pretreatment with NLRP3 and caspase-1 inhibitors such as MCC950, BHB, and VX-765 protects against both food allergen- and aeroallergen-induced EoE pathogenesis in an EoE experimental model.
Finding the hidden needle: a workflow for 3D cryo-correlative light and electron microscopy volume imaging
Imagine capturing 3D images of whole tissue in an unperturbed hydrated state, transitioning from 3D cryo-light microscopy to 3D cryo-electron microscopy. Now this is possible with our 3D cryoCLEM workflow.
Circular Vectors better than plasmids for RNA guide delivery and expression in cell culture experiments
We have developed an efficient, fully synthetic method and protocol called the Circular Vector method, which enables the production of circularized DNA containing expression elements that are ready for transfection in as little as 3 hours, thereby eliminating all bacterial cloning steps.
Meet our authors from RIKEN Bioresource Research Center, Meijo University, and the National Institute of Advanced Industrial Science and Technology in Japan to learn about their fascinating invention for improving pluripotent stem cell culturing.