We have developed an efficient, fully synthetic method and protocol called the Circular Vector method, which enables the production of circularized DNA containing expression elements that are ready for transfection in as little as 3 hours, thereby eliminating all bacterial cloning steps.

Meet our authors from RIKEN Bioresource Research Center, Meijo University, and the National Institute of Advanced Industrial Science and Technology in Japan to learn about their fascinating invention for improving pluripotent stem cell culturing.