Nature Methods
This journal is a forum for the publication of novel methods and significant improvements to tried-and-tested basic research techniques in the life sciences.
SPARK: finding genes with spatial expression patterns
We present Spatial PAttern Recognition via Kernels (SPARK) as an effective statistical tool to identify genes with spatial expression patterns in spatially resolved transcriptomic studies. A particular feature of SPARK is its ability to produce calibrated p-values for spatial analysis.
Reduction of the illumination power for high-speed multiphoton microscopy
An adaptive excitation source enables two- and three-photon imaging of the awake mouse brain with high spatial and temporal resolution at 30-fold-reduced laser power relative to conventional approaches.
The search for a better protein marker of autophagy
Measuring ATG16L1 phosphorylation as an alternative method to monitor autophagy induction
Time-Resolved Serial Femtosecond Crystallography at the European XFEL
The European XFEL (EuXFEL) is an X-ray source that produces femtosecond X-ray pulses at megahertz repetition rates. Time-resolved crystallographic investigations on biological reactions constitute an important class of experiments. We demonstrate how such a reaction is followed at the EuXFEL.
Vascularized Brain Organoids
Genetic engineering to form vascular-like structures within cortical organoids (vhCOs).