In our Nature Protocol, we present step by step instructions on how to make phosphatase inhibitor beads, enrich lysates for phosphoprotein phosphatases, multiplex, phosphopeptide enrich, and analyze samples by mass spectrometry to systematically study the major drivers of protein dephosphorylation.
Raman spectroscopy is an essential technique of process analytical technologies used in the chemical and biopharmaceutical industry for uninterrupted monitoring of manufacturing processes. Now, it is also a powerful tool for monitoring solid-state milling reactions on vibratory ball mills.
Early detection of cancer is key to better outcomes for patients, and cancer screening using minimally invasive samples such as blood, urine, saliva and exfoliated cells is receiving increasing attention.
Pregnancy loss is a devastating event and whilst conventional autopsy techniques can provide parents with answers as to the cause of their loss, many decline due to its invasive nature. We describe micro-CT as an alternative to conventional autopsy and why this is more acceptable to parents.
Systematic assays suitable for use with peptide arrays are described in this protocol to evaluate biological activities associated with host defense peptides (HDPs), such as biofilm inhibition and/or immunomodulatory properties.
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