We have detailed a method for profiling DNA methylation and chromatin accessibility state from a single fragment library. This joint-profiling method, termed ATAC-Me, can now be applied in diverse cellular models to shed light on the complicated relationship between these two epigenetic features.
In our Nature Protocol, we present step by step instructions on how to make phosphatase inhibitor beads, enrich lysates for phosphoprotein phosphatases, multiplex, phosphopeptide enrich, and analyze samples by mass spectrometry to systematically study the major drivers of protein dephosphorylation.
Raman spectroscopy is an essential technique of process analytical technologies used in the chemical and biopharmaceutical industry for uninterrupted monitoring of manufacturing processes. Now, it is also a powerful tool for monitoring solid-state milling reactions on vibratory ball mills.
Early detection of cancer is key to better outcomes for patients, and cancer screening using minimally invasive samples such as blood, urine, saliva and exfoliated cells is receiving increasing attention.
Pregnancy loss is a devastating event and whilst conventional autopsy techniques can provide parents with answers as to the cause of their loss, many decline due to its invasive nature. We describe micro-CT as an alternative to conventional autopsy and why this is more acceptable to parents.
You can consent to the use of such technologies by closing this notice.
Customise your preferences for any tracking technology
The following allows you to customize your consent preferences for any tracking technology used
to help us achieve the features and activites described below. To learn more about how these trackers help us
Register to Springer Nature Protocols and Methods Community
Springer Nature Protocols and Methods Community is a hub for all those who have an interest in methods and protocols, including the editors and authors of SN Experiments, Nature Methods, Nature Protocols and SpringerProtocols