We present Spatial PAttern Recognition via Kernels (SPARK) as an effective statistical tool to identify genes with spatial expression patterns in spatially resolved transcriptomic studies. A particular feature of SPARK is its ability to produce calibrated p-values for spatial analysis.
An adaptive excitation source enables two- and three-photon imaging of the awake mouse brain with high spatial and temporal resolution at 30-fold-reduced laser power relative to conventional approaches.
Measuring ATG16L1 phosphorylation as an alternative method to monitor autophagy induction
Contributor Nature Methods
The European XFEL (EuXFEL) is an X-ray source that produces femtosecond X-ray pulses at megahertz repetition rates. Time-resolved crystallographic investigations on biological reactions constitute an important class of experiments. We demonstrate how such a reaction is followed at the EuXFEL.
Self-coalescence occurs when a liquid is stretched and made to fold onto itself. Using microfluidics, their unique properties can be used to engineer and deliver precise sequences of reagents to realize complex portable bioassays.
Rational design and methods of analysis for the study of short- and long-term dynamic responses of eukaryotic networks
Dynamic response of eukaryotes provide us with a signature of their response to stress, perturbations, sustained, cyclic, or periodic variations and fluctuations, and therefore the analysis of dynamic eukaryotic networks is elemental in achieving a mechanistic understanding of cellular behavior.
Extracting mechanistic details from biomolecular simulations: The case of Watson-Crick to Hoogsteen DNA base pairing transition pathways