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SCCAF: machine learning infers putative cell types
We describe SCCAF a computational approach to identify putative cell clusters from single-cell RNA-seq data. SCCAF automatically identifies the “ground truth” cell assignments with high accuracy in various benchmark datasets and captures the discriminative feature genes of the cell types.
CRaftID: An easy-to-use platform for imaging-based CRISPR screens
We modified the commercially available microraft array technology with standard confocal imaging techniques to perform an imaging-based CRISPR screen for regulators of a protein localization phenotype.
KAS-seq: capturing global transcription activity with kethoxal-assisted labeling
We describe a kethoxal-assisted single-stranded DNA sequencing (KAS-seq) approach. KAS-seq allows rapid (within 5 min), sensitive and genome-wide capture and mapping of ssDNA produced by transcriptionally active RNA polymerases or other processes in situ using as few as 1,000 cells.
Spatial Heterogeneity in Molecular Brightness
Oligomerisation of membrane proteins remains a field characterized by intense research interest, due to the large number of pharmacological and clinical implications that the formation of molecular complexes of signaling proteins carries.
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SCCAF: machine learning infers putative cell types
We describe SCCAF a computational approach to identify putative cell clusters from single-cell RNA-seq data. SCCAF automatically identifies the “ground truth” cell assignments with high accuracy in various benchmark datasets and captures the discriminative feature genes of the cell types.
CRaftID: An easy-to-use platform for imaging-based CRISPR screens
We modified the commercially available microraft array technology with standard confocal imaging techniques to perform an imaging-based CRISPR screen for regulators of a protein localization phenotype.